Analytical probing of membranotropic effects of antimicrobial copper nanoparticles on lipid vesicles as membrane models, Nanoscale Advances 5, 6533-6541 (2023)

In this work, phosphatidylcholine-based large unilamellar vesicles have been explored as mimics of cellular membranes to investigate the effect of ultra-small CuNPs on the physicochemical features of phospholipid membranes. 4 nm-sized CuNPs were synthesized by a wet-chemical route that involves glutathione as a stabilizer, with further characterization by UV-vis absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) spectroscopy. Two fluorescent membrane probes bearing naphthalene moieties (laurdan and prodan) were used to monitor the bilayer structure and dynamics, as well as to demonstrate the strong membranotropic effects of CuNPs. The fluorescence spectroscopic studies were supported by dynamic light scattering (DLS) measurements and the calcein leakage assay. Additionally, the degree of perturbation of the phospholipid bilayer by CuNPs was compared against that of Cu²⁺ ions, the latter resulting in negligible effects. The findings suggested that CuNPs are able to damage the phospholipid membranes, leading to their agglomeration or disruption.